Yale Bulletin and Calendar

November 11, 2005|Volume 34, Number 11

Parasite that causes Sleeping Sickness yields new information about how an organ of the cell duplicates

Researchers at Yale have brought to light a mechanism that regulates the way an internal organelle, the Golgi apparatus, duplicates as cells prepare to divide, according to a report in the Oct. 27 Science Express.

Graham Warren, professor of cell biology, and his colleagues at Yale study Trypanosoma brucei, the parasite that causes Sleeping Sickness. Like many parasites, it is exceptionally streamlined and has only one of each internal organelle, making it ideal for studying processes of more complex organisms that have many copies in each cell.

When studying how cells divide, doubling and separating DNA in chromosomes is often the focus. Equally important to scientists is the way a cell prepares its internal organelles for distribution. Warren studies the Golgi complex, a membrane compartment in the cytoplasm that delivers newly made proteins to different membranes in the cell.

"Basal bodies in particular and centrosomes in general have been implicated in the biogenesis of a number of membrane-bound organelles," says Warren. "It prompted us to study further their role in Golgi duplication."

Warren's group has identified a new cellular structure, distinct from the basal body, involved in the duplication of the Golgi apparatus and defined by a highly conserved protein, Centrin2. This structure has two lobes -- one at the old Golgi, the other where the new Golgi forms. Once a new Golgi has grown, the Centrin structure itself duplicates so that two complete structures, and associated Golgi, are ready to be allocated to daughter cells.

Significant recent advances in the molecular genetics of trypanosomes by Elisabetta Ullu and Christian Tschudi's group at Yale allowed direct manipulation of protein levels using the innate RNA interference (RNAi) system. The relationship between the growing Golgi, the Centrin proteins and other cellular organelles was shown in experiments using RNAi, and visualizing the process was possible with fluorescent protein tags. How this process relates to higher organisms is the focus of present research.

Cynthia Y. He and Marc Pypaert of Yale were co-authors on the work, which was funded by the National Institutes of Health.

-- By Janet Rettig Emanuel

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